SIMULTANEOUS HPLC DETERMINATION OF METABOLITES OF THE INFLAMMATORY CASCADE

Objective: A model approach is presented to the in vivo inflammation c ascade, in which the activities of key enzymes (phospholipase A(2) [3. 1.1.4], prostaglandin synthase [EC 1.14.99.1], and lipoxygenases [EC 1 .13.11.12]) are determined simultaneously in a single in vitro assay. Methods and Results: Detection of phospholipids (up to 50 pM) and arac hidonic acid (up to 33 pM) is attained with high sensitivity and witho ut radiolabelling using a SEDERE light scattering detector. Conclusion s: Thus, in combination with a diode array UV-detector, lipids, prosta glandins, HETEs and other metabolites of the inflammation cascade can be determined with high efficiency using a reversed phase-high perform ance liquid chromatograph equipped with two highly sensitive detectors in series.