GIBBERELLIN A(3) CAUSES A DECREASE IN THE ACCUMULATION OF MESSENGER-RNA FOR ACC OXIDASE AND IN THE ACTIVITY OF THE ENZYME IN AZUKI-BEAN (VIGNA-ANGULARIS) EPICOTYLS
T. Kaneta et al., GIBBERELLIN A(3) CAUSES A DECREASE IN THE ACCUMULATION OF MESSENGER-RNA FOR ACC OXIDASE AND IN THE ACTIVITY OF THE ENZYME IN AZUKI-BEAN (VIGNA-ANGULARIS) EPICOTYLS, Plant and Cell Physiology, 38(10), 1997, pp. 1135-1141
Differential screening, aimed at the isolation of cDNA clones of mRNAs
whose accumulation is influenced by GA(3), resulted in the isolation
of a cDNA clone of an mRNA whose level was decreased by GA(3) in segme
nts of epicotyls of Vigna angularis. The putative protein encoded by t
his cDNA resembled the 1-aminocyclopropane-1-carboxylate oxidases (ACC
oxidases) identified in other plant species (about 80% homology at th
e amino acid level). Thus, the corresponding gene was designated AB-AC
O1 (azuki bean ACC oxidase). GA(3) also decreased the activity of ACC
oxidase in azuki bean epicotyls, but it did not decrease the rate of e
thylene evolution. In fact, GA(3) increased the rate of ethylene evolu
tion and the level of ACC. Thus, GA(3) seemed to increase the producti
on of ethylene by promoting the synthesis of ACC.