18 NEW POLYMORPHIC MARKERS IN THE MULTIPLE ENDOCRINE NEOPLASIA TYPE-1(MEN1) REGION

Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant di sorder in which affected individuals develop tumors primarily in the p arathyroids, anterior pituitary, endocrine pancreas, and duodenum. The locus fur MEN1 is tightly linked to the marker PYGM on chromosome 11q 13, and linkage analysis has previously placed the MEN1 gene within a 2-Mb Interval flanked by markers D11S1883 and D11S449. Loss of heteroz ygosity (LOH) studies in MEN1 and sporadic tumors have helped narrow t he location of the gene to a 600-kb interval between PYGM and D11S449. Eighteen new polymerase chain reaction (PCR)-based polymorphic marker s were generated for the MEN1 region, with ten mapping to the PYGM-D11 S449 interval. These new markers, along with 14 previously known polym orphic markers, were precisely mapped on a 2.8-Mb (D11S480-D11S913) hi gh-density clone contig-based, physical map generated for the MEN1 reg ion.