MECHANISMS OF CYCLOHEXIMIDE-INDUCED APOPTOSIS IN LIVER-CELLS

Cycloheximide in sublethal doses caused apoptosis in liver cells in vi vo, inducing c-myc, c-fos, c-jun and p53 genes and accumulation of sph ingosine, a toxic product of the sphingomyelin cycle, These data suppo rt the hypothesis that continuous synthesis of labile protective prote ins is required to restrain apoptosis in liver; sphingosine might be i mportant in mediating cycloheximide-induced apoptosis as an endogenous modulator of protein kinase C activity. (C) 1997 Federation of Europe an Biochemical Societies.