COMPRESSIBILITY AND VOLUME CHANGES OF LYSOZYME DUE TO GUANIDINE-HYDROCHLORIDE DENATURATION

Authors
KAMIYAMA T GEKKO K
Citation
T. Kamiyama et K. Gekko, COMPRESSIBILITY AND VOLUME CHANGES OF LYSOZYME DUE TO GUANIDINE-HYDROCHLORIDE DENATURATION, Chemistry Letters, (10), 1997, pp. 1063-1064
Citations number
14
Categorie Soggetti
Chemistry
Journal title
Chemistry Letters → ACNP
ISSN journal
03667022
Issue
10
Year of publication
1997
Pages
1063 - 1064
Database
ISI
SICI code
0366-7022(1997):10<1063:CAVCOL>2.0.ZU;2-I
Abstract
The apparent specific volume and adiabatic compressibility of lysozyme largely decreased upon guanidine hydrochloride denaturation. These re sults demonstrate that a large amount of amino acid residues exposes a ccompanying an increase in hydration and a decrease in the internal ca vity. Both parameters deviated from the two-state transition curves, s uggesting noncooperative disruption of the compactness and the seconda ry structure.