DEVELOPMENT AND ANALYTICAL PERFORMANCE OF AN AUTOMATED SCREENING METHOD FOR CANNABINOID ON THE DIMENSION(R) CLINICAL-CHEMISTRY SYSTEM

A fully automated:, random access method for the determination of cann abinoids (UTHC) was developed for the Dimension((R)) AR and XL clinica l chemistry systems. The method utilizes Abuscreen ONLINE(R) reagents and a multianalyte liquid calibrator containing 11-nor-Delta(9)-THC-9- carboxylic acid. Within-run and total reproducibility, determined usin g NCCLS protocol EP5-T2, was less than 0.6% and 1.6% CV, respectively, at all concentrations. Calibration stability was retained for at leas t 30 days. An extensive evaluation of non-structurally related drugs a nd various physiological substances indicated lack of interference in the method. No sample carry-over was observed following a specimen con taining 1886 ng/ml 11-nor-Delta(9)-THC-9-carboxylic acid. A 99.1% agre ement (N = 445 samples) was Sound between an EMIT(R) based method on t he aca((R)) discrete clinical analyser and the Dimension((R)) UTHC met hod. Dimension((R)) clinical chemistry system and aca((R)) discrete cl inical analyser are registered trademarks of Dade International.