Lp. Ammann et al., POWER, SAMPLE-SIZE AND TAXONOMIC SUFFICIENCY FOR MEASURES OF IMPACT IN AQUATIC SYSTEMS, Environmental toxicology and chemistry, 16(11), 1997, pp. 2421-2431
As part of a larger program to examine the relationships between labor
atory and ambient toxicity, data from a series of 28 marine microcosm
experiments, which included both laboratory and held studies, were rea
nalyzed. The statistical methods applied to the data include log-linea
r regression models (general and robust) for richness and taxa count d
ata (kingdom, phylum, class, order, family, genus, and species) and Ga
ussian linear models (general and robust) for community metrics divers
ity and evenness (Shannon-Weiner's, Simpson's, and Brillouin's) and si
milarity (Bray-Curtis). In addition, taxonomic sufficiency was determi
ned for each of the metrics examined. For each of the analyses perform
ed, both the statistical power of the analysis and the sample sizes re
quired to achieve a desired level of power were determined. New method
s are presented to deal with zero count data. Suggestions on how the p
ower of microcosm tests used in toxicity testing can be increased are
provided. The statistical programs developed for the analyses applied
to the data analyzed were done in S-PLUS. The programs and directions
for their application have been placed on an anonymous FTP site at the
University of North Texas.