SULFOQUINOVOSYL DIACYLGLYCEROLS FROM THE ALGA HETEROSIGMA CARTERAE

An extract of the chloromonad Heterosigma carterae (Raphidophyceae), c ultivated in natural seawater, contained a complex mixture of sulfoqui novosyl diacylglycerols. Palmitoyl (16:0), three isomers of hexadeceno yl (16:1 cis Delta 9, Delta 11, Delta 13), and eicosapentenoyl (20:5) were found to be the main fatty acyl substituents. Exact double-bond s ites were determined by mass spectrometry analysis of the correspondin g nicotinyl derivatives. Four major sulfoquinovosyl diacylglycerol com ponents were partially purified and identified as 1-4 by interpretatio n of their nuclear magnetic resonance and mass spectral data. In addit ion, complete analysis of the H. carterae sulfoquinovosyl diacylglycer ols was performed using high-performance liquid chromatography combine d with electrospray tandem mass spectrometry.