EFFECTS OF SEROTONIN AND ENDOTHELIN ON THE SMOOTH-MUSCLE CELLS OF AUTOGENOUS VEIN GRAFTS

Citation
K. Mawatari et al., EFFECTS OF SEROTONIN AND ENDOTHELIN ON THE SMOOTH-MUSCLE CELLS OF AUTOGENOUS VEIN GRAFTS, British Journal of Surgery, 84(10), 1997, pp. 1419-1424
Citations number
43
Categorie Soggetti
Surgery
Journal title
ISSN journal
00071323
Volume
84
Issue
10
Year of publication
1997
Pages
1419 - 1424
Database
ISI
SICI code
0007-1323(1997)84:10<1419:EOSAEO>2.0.ZU;2-O
Abstract
Background The purpose of this study was to compare the effects of vas oconstrictor substances such as 5-hydroxytryptamine (5-HT) and endothe lin on the smooth muscle of canine femoral veins and vein grafts. Meth ods The right canine femoral vein was grafted into the right femoral a rtery. The left femoral vein was used as a control. In other experimen ts to examine the effects of surgical procedures such as dissection of the adventitia and the effects of grafting (vein-to-vein bypass), the right femoral vein was dissected out but not removed for grafting and an autogenous vein bypass of the right femoral vein was made using th e left femoral vein. In all experiments, the veins were removed 4 week s after operation and suspended in organ chambers for isometric tensio n recording. Results Maximum contractions to endothelin were comparabl e in control vein and vein grafts. In control vein, the maximum contra ction to 5-HT was small, and was inhibited by both methiothepin, a 5-H T1 and 5-HT2 antagonist, and sarpogrelate hydrochloride, a 5-HT2 antag onist. In vein grafts 5-HT produced significantly larger contractions than in control veins, which were inhibited by methiothepin but not by the 5-HT2 antagonist. In veins with adventitial dissection alone and vein-to-vein grafts, 5-HT produced small contractions which were compa rable to those in control vein. Conclusion The larger contraction resp onse to 5-HT in canine vein grafts may be due to an increased responsi veness of the 5-HT1 receptor caused by grafting into the arterial circ ulation.