BIOELECTROCHEMICAL 2-PROPANOL BIOSENSORS

2-Propanol biosensors for O-2-detectors based on bienzyme membranes wi th sequentially installed catalase and alcohol oxidase are described. Measurements were performed in undiluted media up to 20 vol.-% 2-propa nol according to the principle of intermediate carrier analysis by use of a micro-dialysator, an oxygenating pump and a pressure adjusting c hamber. For the extension of the measuring scale in the enzyme membran e O-2 is released out of hydrogen peroxide within the phosphate buffer ed carrierstream by catalase. Therefore, oxygen is available to the se rially installed alcohol oxidase for the enzymatic substrate transform ation beyond the physically soluted part of oxygen in the measuring me dium. Molecular selectivities of bienzyme membranes are described for normally used moisturizing concentrates in printeries.