HEPATOTOXICITY OF 1,3,5-TRINITRO-2-ACETYL PYRROLE DERIVED FROM NITROSATION OF MAILLARD REACTION-PRODUCT IN BALB C MOUSE/

1,3,5-Trinitro-2-acetyl pyrrole (TNAP) is a product derived from the r eaction of 2-acetyl pyrrole with nitrite in the model of Maillard brow ning systems. This compound is moderately mutagenic to the Salmonella strains TA98 and TA100 and is markedly cytotoxic to mouse C3H10T1/2 ce lls. Experiments are performed to investigate the effects of TNAP on t he hepatic toxicity in mouse. Male BALB/C mice were subjected to a dos e of 7.2 mg/kg body weight twice a week by i.p. injection for 24 weeks , then followed by a feeding diet for 21 weeks. TNAP-treated mice show ed an increase in mortality and time-dependent appearance of lesions i n the liver. TNAP is hepatotoxic as demonstrated by a marked increase in the activities of serum alanine transaminase (ALT) and aspartic tra nsaminase (AST). TNAP-related lesions observed histologically in mice, included hapatic atrophy, mild fatty metamorphosis with multilocular cysts in the liver. In conclusion, TNAP was considered to be a toxic c ompound in mice as evidenced by increased incidences of mortality, and lesions of liver.