SUPPRESSION OF MICROSOMAL CYTOCHROME P450-DEPENDENT MONOOXYGENASES AND MITOCHONDRIAL OXIDATIVE-PHOSPHORYLATION BY FULLERENOL, A POLYHYDROXYLATED FULLERENE C-60
Th. Ueng et al., SUPPRESSION OF MICROSOMAL CYTOCHROME P450-DEPENDENT MONOOXYGENASES AND MITOCHONDRIAL OXIDATIVE-PHOSPHORYLATION BY FULLERENOL, A POLYHYDROXYLATED FULLERENE C-60, Toxicology letters, 93(1), 1997, pp. 29-37
The acute toxicity of fullerenol-1 was determined using mice pretreate
d intraperitoneally (i.p.) with polyhydroxylated C-60 derivatives. The
LD50 value of fullerenol-1 was estimated to be 1.2 g/kg. Pretreatment
s with 0.5 and 1.0 g/kg fuIlerenol-1 decreased cytochromes P450 and b(
5) contents, and NADPH-cytochrome P450 reductase, benzo[a]pyrene hydro
xylase, 7-ethoxycoumarin O-deethylase, aniline hydroxylase, and erythr
omycin N-demethylase activities in liver microsomes. Pretreatments wit
h 0.01 and 0.1 g/kg fullerenol-1 had no effect on these monooxygenases
. Additions of fullerenol-1 to mouse liver microsomes suppressed monoo
xygenases activities toward benzo[a]pyrene, 7-ethoxycoumarin, aniline,
and erythromycin with IC50 values of 42, 94, 102 and 349 mu M, respec
tively. Fullerenol-1 exhibited noncompetitive and mixed-type of inhibi
tion in benzo[a]pyrene hydroxylation and 7-ethoxycoumarin O-deethylati
on, respectively. Additions of fullerenol-1 to rat liver mitochondria
resulted in a dose-dependent inhibition of ADP-induced uncoupling and
markedly inhibited mitochondrial Mg2+-ATPase activity with an IC50 val
ue of 7.1 mu M. These results demonstrate that fullerenol-1 can suppre
ss the levels of the microsomal enzymes in vivo and decrease the activ
ities of P450-dependent monooxygenase and mitochondrial oxidative phos
phorylation in vitro. (C) 1997 Elsevier Science Ireland Ltd.