S. Simcic et al., LOCAL CHANGES IN MEMBRANE-POTENTIAL INTENSIFY NEUTROPHIL OXIDATIVE BURST, Physiological chemistry and physics and medical NMR, 29(1), 1997, pp. 39-47
Citations number
25
Categorie Soggetti
Biophysics,Biology,Physiology,"Radiology,Nuclear Medicine & Medical Imaging
The aim of this paper is to study the effects of the pulsed electrical
field/current alone or combined with ionomycin, fMLP and PMA, the che
mical stimuli that operate through distinctly different activation pat
hways, on the time course of the oxidative burst response in human neu
trophils. Neither the control groups nor the neutrophils treated with
electrical field alone shelved any increase in oxidative burst activit
y measured by the luminol-enhanced chemiluminescence technique, It was
found that electrical treatment potentiates chemically induced activa
tion with either of the chemical stimulators used. The integrated oxid
ative burst response-which represents a cumulative amount of oxygen me
tabolites produced during whole response-was 87% higher in neutrophils
treated with a combination of ionomycin and an electric field than in
solely ionomycin treated cells, while the peak level of the response
was 114% higher. In neutrophils stimulated with fMLP the electrochemic
al treatment caused a 32% higher integral response as well as a 22% hi
gher peak level compared to the neutrophils treated with chemical stim
ulant alone, The integrated oxidative burst response in the combined P
MA and electric treatment was only 4.7% higher than in the cells treat
ed with PMA alone, and no significant difference in the peak level was
found, The results suggest that electric field treatment preferential
ly stimulates calcium-induced activation with ionomycin rather than ca
lcium-dependent activation with fMLP or PMA.