USE OF A RAPID METHOD FOR GENOTYPING HUMAN PLATELET ANTIGEN SYSTEMS IN NEONATAL ALLOIMMUNE THROMBOCYTOPENIA

We applied a polymerase chain reaction-sequence specific primer (PCR-S SP) method developed by other researchers to study 4 families of newbo rns with neonatal alloimmune thrombocytopenia (NAITP) in which serolog y had provided inconclusive human platelet antigen (HPA) typing data. This method allowed for the identification of the newborn HPAs which w ere incompatible with their respective mothers. They were HPA-2b, -1b, -3a, and -5b. This PCR-SSP is a useful tool for improving the ability to identify the incompatible HPA in NAITP. (C) 1997, Ferrata Storti F oundation.