M. Karbasi et T. Keshavarz, EFFECT OF DILUTION RATE ON THE STABILITY OF TOL PLASMID PTK0 IN PSEUDOMONAS-PUTIDA PPK1, Biotechnology letters, 19(10), 1997, pp. 985-988
Continuous culture fermentation of Pseudomonas putida PPK1, containing
the naturally occurring TOL plasmid pTK0, was carried out in either b
enzoate- or succinate-limited chemostats. The apparent stability of th
e plasmid decreased with increasing the dilution rate. An established
model was used to predict the contribution of segregational instabilit
y and growth rate difference to the apparent plasmid stability.