Cultures of Phanerochaete chrysosporium, operating with an inert carri
er (nylon sponge) and a non-inert carrier (barley straw), were employe
d in order to study laccase production during semi-solid state conditi
ons. Manganese (IV) oxide, added to the cultures increased laccase act
ivity 16-fold especially in barley straw cultures, in which a maximum
laccase activity of 360 U/l (one unit is defined as 1 mu mol of 2'-azi
no-di-[3-ethyl-benzothiazoline-(6)-sulphonic acid] oxidized per minute
) was achieved.