HYDROLYSIS OF 2-SUBSTITUTED ARYL AND HETEROARYL ALKANOATES BY CANDIDA-RUGOSA LIPASE

Authors
GARCIA M GRADILLAS A DELCAMPO C LLAMA EF SANCHEZMONTERO JM SINISTERRA JV
Citation
M. Garcia et al., HYDROLYSIS OF 2-SUBSTITUTED ARYL AND HETEROARYL ALKANOATES BY CANDIDA-RUGOSA LIPASE, Biotechnology letters, 19(10), 1997, pp. 999-1004
Citations number
18
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
19
Issue
10
Year of publication
1997
Pages
999 - 1004
Database
ISI
SICI code
0141-5492(1997)19:10<999:HO2AAH>2.0.ZU;2-B
Abstract
The hydrolysis of several esters catalyzed by lipase of Candida rugosa was used to analyse the interaction between the ester substrate and t he active site and the geometry and steric restrictions of 'alk', 'ar' and 'h' subsites. Using 4-R-C6H4-CH(R')-COOEt and ethyl trans-cinnama te, the presence of a low electronic density zone in the `ar' pocket i s described, the dimensions of the pocket produced by Phe-296 and Leu- 297 in the `alk' subsite and the size of the 'h' subsite are described .