CLONING AND NUCLEOTIDE-SEQUENCE OF A THERMOSTABLE CYCLODEXTRIN GLYCOSYLTRANSFERASE GENE FROM THERMOANAEROBACTER SP. ATCC-53627 AND ITS EXPRESSION IN ESCHERICHIA-COLI

Authors
JORGENSEN ST TANGNEY M STARNES RL AMEMIYA K JORGENSEN PL
Citation
St. Jorgensen et al., CLONING AND NUCLEOTIDE-SEQUENCE OF A THERMOSTABLE CYCLODEXTRIN GLYCOSYLTRANSFERASE GENE FROM THERMOANAEROBACTER SP. ATCC-53627 AND ITS EXPRESSION IN ESCHERICHIA-COLI, Biotechnology letters, 19(10), 1997, pp. 1027-1031
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
19
Issue
10
Year of publication
1997
Pages
1027 - 1031
Database
ISI
SICI code
0141-5492(1997)19:10<1027:CANOAT>2.0.ZU;2-H
Abstract
A gene, cgtA, encoding an extremely thermostable cyclodextrin glycosyl transferase (CGTase) was cloned from a thermophilic anaerobe, Thermoan aerobacter sp. ATCC 53627, and expressed in Escherichia coli. DNA and protein sequencing revealed that the mature enzyme of 683 amino acid r esidues (MW 75 kDa) was preceded by a signal peptide of 27 amino acid residues. The sequence of the Thermoanaerobacter CGTase was similar to sequences of Bacillus CGTases, with more than 58% identity, and very similar (89% identity) to a CGTase enzyme from Thermoanaerobacterium t hermosulfurogenes.