DISTRIBUTION OF CHERRY LEAF ROLL NEPOVIRUS (CLRV) IN LEAVES OF DECIDUOUS FOREST TREES AND HERBACEOUS PLANTS DETECTED BY TISSUE PRINT IMMUNOPRESSBLOTTING (TPI) OF WHOLE LEAF BLADES

Distribution and localization of cherry leaf roll nepovirus (CLRV) in leaves of deciduous forest trees and herbaceous hosts revealing chloro tic specs, line patterns and chlorotic areas, were investigated by tis sue print immunopressblotting (TPI). The routine preparation method fo r rolled leaf blades had to be modified for detection of virus distrib ution patterns in petals. This was achieved by mechanical removal of t he lower epidermis and press blotting the leaves onto nitrocellulose i n a vise. In Betula pendula- Roth and Prunus avium L.-leaves, detectio n of virus antigen generally was confined to chlorotic specs and line patterns. Virus distribution in herbaceous hosts varied depending on h ost plane species: In leaves of Nicotiana tabacum L. cv. 'Xanthi-nc', viral antigen could only be detected in chlorotic or necrotic patterns as described for woody plants, whereas in Chenopodium quinoa Willd.-l eaves, virus was also present in green areas. The results were confirm ed by ELISA.