Gh. Borchert et al., MOLECULAR-FORMS OF PROSTATE-SPECIFIC ANTIGEN IN THE SERUM OF WOMEN WITH BENIGN AND MALIGNANT BREAST DISEASES, British Journal of Cancer, 76(8), 1997, pp. 1087-1094
Using a highly sensitive immunofluorometric procedure, we measured the
total prostate-specific antigen (PSA) concentration in 632 sera obtai
ned from female blood donors and women with idiopathic hirsutism, brea
st cancer or benign breast diseases. A total of 50 sera with total PSA
> 15 ng l(-1) were fractionated by high-performance liquid chromatogr
aphy (HPLC) in order to resolve the two immunoreactive molecular forms
, i.e. free PSA (approximately 30 kDa) and PSA bound to alpha(1)-antic
hymotrypsin (PSA-ACT, 100 kDa). We found that breast cancer patients h
ave presurgical serum total PSA levels similar to!hose of blood donors
. Total serum PSA concentration decreases with age in women with idiop
athic hirsutism, in cancer patients and in patients with benign breast
diseases. The major molecular form of PSA in the serum of all normal
and hirsute women (n = 15) is PSA bound to the proteinase inhibitor al
pha(1)-antichymotrypsin. The major molecular form in 44% of presurgica
l cancer patient sera-is free PSA. A total of 58% of benign breast dis
ease patients also have in their serum mainly free PSA. We conclude th
ai about half the patients with breast cancer or benign breast disease
s have free PSA as the major molecular form in their serum, whereas pa
tients without breast pathologies (normal blood donors, idiopathic hir
sutism) have PSA bound to alpha(1)-antichymotrypsin as the major molec
ular form. The ratio of PSA/PSA-ACT may have Value as a simple biochem
ical test for diagnosis of breast pathologies including breast cancer.