ANAEROBIC NITRATE AND AMMONIUM METABOLISM IN FLOOD-TOLERANT RICE COLEOPTILES

The tolerance of germinating rice seedlings to anaerobiosis cannot be fully accounted for by ethanolic fermentation alone. Nitrate metabolis m (nitrate reduction to NH4+ plus its subsequent assimilation) may pro vide an additional sink mechanism for excess protons and NADH produced during anaerobiosis. To follow the fate of nitrate, N-15-labelled nit rate and ammonium incorporation in aerobic and anaerobic rice coleopti les was examined using N-15-edited H-1 NMR and gas chromatography-mass spectrometry methods. After 22 h of treatments, protein-free Ala, Glu , Gin, and gamma-aminobutyrate were the main N-15-labelled products fo r both nitrate and ammonium-treated anaerobic rice coleoptiles, with G in, Glu, and Ala being the most enriched, The total amount of N-15 lab el incorporation into Ala and GAB increased significantly in response to anaerobiosis, The N-15-labelling pattern of Glu and Gin suggests th at the GS/GOGAT system was primarily involved in ammonium assimilation whereas Glu dehydrogenase may play a role in nitrate assimilation. N- 15 incorporation into protein-derived amino acids was also significant and was more substantial in anaerobic than in aerobic rice coleoptile s, which indicate that protein biosynthesis remained active in anaerob ic rice coleoptiles, Thus, anaerobic assimilation of inorganic N into amino acids, particularly Ala and Glu/GAB, may serve to supplement eth anolic fermentation in sustaining glycolysis and energy production in rice coleoptiles.