THE EFFECT OF CHORIONIC VILLUS SAMPLING ON THE NUMBER OF FETAL CELLS ISOLATED FROM MATERNAL BLOOD AND ON MATERNAL SERUM ALPHA-FETOPROTEIN LEVELS

Fetal cells are present in the circulation of pregnant women and can b e isolated using density gradient centrifugation and magnetic cell sor ting. In the present study, maternal cell preparations were depleted f or CD45- and CD14-positive cells and enriched for CD14-positive cells. The number of fetal nucleated cells was determined using fluorescence in situ hybridization for X and Y chromosomes. Analysis of maternal b lood samples taken before and after transabdominal chorionic villus sa mpling (TA-CVS) showed an increase in the number of fetal cells in 10 out of 19 male pregnancies after the invasive procedure. This Cellular transfusion was found to correlate with elevated maternal serum alpha -fetoprotein levels. TA-CVS-induced cellular transfusion may form a go od in vivo system to optimize fetal cell isolation procedures and to s tudy fetal cell dynamics and characteristics. (C) 1997 by John Wiley & Sons, Ltd.