EXPRESSION PROFILES OF TRANSCRIPTS FROM 126 OPEN READING FRAMES IN THE ENTIRE CHROMOSOME-VI OF SACCHAROMYCES-CEREVISIAE BY SYSTEMATIC NORTHERN ANALYSES

Chromosome VI of Saccharomyces cerevisiae contains 126 open reading fr ames (ORFs), and the functions of proteins encoded by 80 ORFs are stil l unknown. In this report, we have systematically examined the express ion profiles of all 126 ORFs on chromosome VI under five kinds of grow th conditions by quantitative Northern hybridization. A series of Nort hern analyses and reverse transcription polymerase chain reactions hav e revealed that more than 64 novel ORFs are transcribed. Two ORFs (YFL 059w and YFR011c) are specifically expressed in the presence of galact ose. Two ORFs (YFL012w and YFR032c) are specifically transcribed in sp orulation. Six ORFs (YFL049w, YFL035c, YFL010c, YFR006w, YFR010w and Y FR017c) are abundantly expressed in many growth conditions. (C) 1997 J ohn Wiley & Sons, Ltd.