ISOLATION AND CHARACTERIZATION BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY AND HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY OF OLIGOSACCHARIDES DERIVED FROM HYALURONIC-ACID BY HYALURONATE LYASE DIGESTION - OBSERVATION OF SOME HERETOFORE UNOBSERVED OLIGOSACCHARIDES THAT CONTAIN AN ODD NUMBER OF UNITS
Kn. Price et al., ISOLATION AND CHARACTERIZATION BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY AND HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY OF OLIGOSACCHARIDES DERIVED FROM HYALURONIC-ACID BY HYALURONATE LYASE DIGESTION - OBSERVATION OF SOME HERETOFORE UNOBSERVED OLIGOSACCHARIDES THAT CONTAIN AN ODD NUMBER OF UNITS, Carbohydrate research, 303(3), 1997, pp. 303-311
Hyaluronic acid was degraded with hyaluronate lyase (E.C. 4.2.2.1, fro
m Streptomyces hyalurolyticus), and the resulting oligosaccharides up
to dp 16 were characterized by electrospray-ionization mass spectromet
ry (ESIMS) and high-performance anion-exchange chromatography (HPAEC)
with pulsed amperometric detection (PAD). In accordance with the known
regiospecificity of the enzyme, the products included even-numbered o
ligosaccharides of structure beta-D-4en-thrHexpA-(1 --> 3)-[beta-D-Glc
pNAc-(1 --> 4)-beta-D-GlcpA](n)-(1 --> 3)-D-GlcpNAc. Minor amounts of
novel and unexpected odd-numbered oligomers, having the structure beta
-D-4en-thrHexpA-(1 --> 3)-[beta-D-GlcpNAc-(1 --> 4)-D-GlcpA](n), were
also isolated and characterized. This study, in addition to others beg
inning to appear in the literature, demonstrates the usefulness of ESI
MS and HPAEC-PAD in the analysis and characterization of anionic glyco
saminoglycan-type oligosaccharides. (C) 1997 Elsevier Science Ltd.