A NEW METHOD TO DETERMINE DNA SUGAR CONFORMATION FROM THE JOINT USE OF 2D AND 3D NMR DATA

The use of sugar restraints has been proven essential for assessing DN A structures through molecular modeling studies. We present a new meth od combining 2D (COSY and NOESY) and 3D (NOESY-NOESY) experiments, whe re constraints on either the phase angles or the difference between ph ase angles of two residues are obtained from comparison of 2D NOE H1'- H4' intensities and 3D NOE intensities containing the H1'-H4' transfer . All experiments lead to restraints that match, proving the validity of the method.