MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY FOR SELECTED TROPANEALKALOID ANALYSIS IN PLANT-EXTRACT

Micellar electrokinetic capillary chromatography was applied to the si multaneous analysis of six tropane alkaloids, including hyoscyamine an d scopolamine. Successful results were obtained using a 30 mM borate-p hosphate buffer at basic pH (8.5) in the presence of 50 mM sodium dode cyl sulfate. The operating conditions, such as buffer concentration an d pH, micelle concentration and organic modifier type and percentage w ere also discussed on the basis of the results given with a tropane al kaloid mixture. Addition of organic modifiers showed an improvement in separation efficiency and resolution. Moreover, hyoscyamine and litto rine, two positional isomers, were only resolved by the addition of or ganic solvents such as methanol or acetonitrile. The optimized conditi ons were finally applied to the analysis of tropane alkaloids found in genetically transformed root cultures of Datura candida x D. aurea.