RAPID ISOCRATIC HPLC ANALYSIS OF BETA-CAROTENE IN RED-PEPPERS (CAPSICUM-ANNUUM L.) AND FOOD PREPARATIONS

Authors
WEISSENBERG M LEVY A SCHAEFFLER I MENAGEM E BARZILAI M
Citation
M. Weissenberg et al., RAPID ISOCRATIC HPLC ANALYSIS OF BETA-CAROTENE IN RED-PEPPERS (CAPSICUM-ANNUUM L.) AND FOOD PREPARATIONS, Chromatographia, 46(7-8), 1997, pp. 399-403
Citations number
30
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Chromatographia → ACNP
ISSN journal
00095893
Volume
46
Issue
7-8
Year of publication
1997
Pages
399 - 403
Database
ISI
SICI code
0009-5893(1997)46:7-8<399:RIHAOB>2.0.ZU;2-6
Abstract
A simple, rapid high-performance liquid chromatography method has been devised in order to separate and quantify beta-carotene present in re d pepper (Capsicum annuum L.) fruits and food preparations. A reversed -phase isocratic non-aqueous system enables the separation of beta-car otene within a few minutes, with detection at 450 nm. The selection of extraction solvents, mild saponification conditions, and chromatograp hic features, is evaluated and discussed. The method is proposed for r apid screening of large plant populations, plant selection, as well as for food products, and also for nutrition and quality control studies .