IMMUNODIAGNOSIS OF TOXOCARA-VITULORUM TISSUE-PHASE INFECTION IN BUFFALOS (BUBALUS-BUBALIS)

Double immunodiffusion (DID), bentonite flocculation (BF) and circumla rval precipitin (CLP) tests were used for detecting tissue-phase Toxoc ara vitulorum infection induced experimentally in buffaloes. Ail these tests detected the antibodies against second stage larval somatic ant igen up to 45 days post-infection (DPI). Being easy to perform and acc urately interpretable, the DID was considered suitable for testing the duration of antibody response in this study. BF test gave positive re sults with the antiserum diluted up to 1:32 and the CLP test showed a maximum titre of 1:8 at 14 DPI. The passive cutaneous anaphylaxis (PCA ) with micro amount of antigen gave positive reaction even when the an tiserum was diluted up to 1:16.