Jh. Lange et Kw. Thomulka, USE OF THE VIBRIO-HARVEYI TOXICITY TEST FOR EVALUATING MIXTURE INTERACTIONS OF NITROBENZENE AND DINITROBENZENE, Ecotoxicology and environmental safety, 38(1), 1997, pp. 2-12
A mixture toxicity investigation was conducted using the bioluminescen
t marine bacterium Vibrio harveyi as the test organism for dual combin
ations of nitrobenzene and dinitrobenzene. Change in bioluminescence w
as used for determination of toxicity, combination toxicity was evalua
ted using statistical comparisons, isopleths (isobologram and isobole
plot), an additive index, and a mixture toxicity index. Both isopleths
and mixture toxicity indices suggest that various combinations are ad
ditive, while the additive index value suggests antagonism. All evalua
tions were conducted as equitoxic mixtures. Statistical determination
was performed using the z test. Numerous comparisons were different at
the 1% level. Slope of line associated with isobole plot was suggeste
d to be an important factor, resulting in statistical differences amon
g comparisons. Distribution, using the Shapiro-Wilk test, was determin
ed for both individual combination groups and solution composition in
isopleths. All distributions evaluated were normal. These results sugg
est that the V. harveyi toxicity test is useful for mixture toxicity s
tudies. (C) 1997 Academic Press.