ASSAY OF ZATEBRADINE IN PLASMA BY FULLY AUTOMATED SAMPLE CLEANUP, CAPILLARY GAS-CHROMATOGRAPHY AND AMMONIA CHEMICAL-IONIZATION MASS-SPECTROMETRY

Authors
SCHMID J BUCHELER A MULLER B
Citation
J. Schmid et al., ASSAY OF ZATEBRADINE IN PLASMA BY FULLY AUTOMATED SAMPLE CLEANUP, CAPILLARY GAS-CHROMATOGRAPHY AND AMMONIA CHEMICAL-IONIZATION MASS-SPECTROMETRY, Journal of chromatography B. Biomedical applications, 658(1), 1994, pp. 93-101
Citations number
7
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
658
Issue
1
Year of publication
1994
Pages
93 - 101
Database
ISI
SICI code
Abstract
A method has been developed for the measurement of zatebradine (UL-FS 49), a heart-rate lowering drug, suitable for the treatment of stable angina pectoris. The method comprises a fully automated liquid-solid e xtraction using a Zymark Benchmate, a capillary gas chromatography and ammonia chemical ionisation (CI) mass spectrometry using hexadeuterat ed zatebradine for the internal standard. The assay has a mean between -batch imprecision of 4.9% and a mean inaccuracy of 1.5%. The calibrat ion curve covers the range of 1-30 ng/ml. About 60 samples can be hand led per day. The assay has been successfully applied to human pharmaco kinetic studies.