HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC MEASUREMENT OF THE NOVEL ANTI-HIV AGENT 7,8-DIHYDROCOSTATOLIDE (NSC-661123)

An HPLC assay was used to determine levels of a promising anti-HIV age nt 7,8-dihydrocostatolide (DC; NSC 661123) in human and murine plasma. The structurally related compound costatolide (C) was found to be a s uitable internal standard. Drug was extracted from human or murine pla sma using a solid-phase C-18 cartridge. The compound was eluted from a n ODS analytical HPLC column using an acetonitrile-water mobile phase. Drug was quantified over the assay range of 19.5 to 625 ng/ml with ex cellent within- and between-day reproducibility. Data resulting from t he use of the assay method for determination of dihydrocostatolide pha rmacokinetics in mice are presented. This is the first report of a val idated HPLC assay for determining DC levels in human and mouse plasma.