LIQUID-CHROMATOGRAPHIC DETERMINATION OF THE ADENOSINE RECEPTOR AGONIST CGS-21680 IN BLOOD USING ONLINE SOLID-PHASE EXTRACTION ON A PHENYLBORONIC ACID SUPPORT AND FLUORESCENCE DETECTION
H. Irth et al., LIQUID-CHROMATOGRAPHIC DETERMINATION OF THE ADENOSINE RECEPTOR AGONIST CGS-21680 IN BLOOD USING ONLINE SOLID-PHASE EXTRACTION ON A PHENYLBORONIC ACID SUPPORT AND FLUORESCENCE DETECTION, Journal of chromatography B. Biomedical applications, 658(1), 1994, pp. 207-212
Citations number
16
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
An analytical method is described for the selective determination of A
, or A, adenosine receptor agonists in blood. By implementing solid-ph
ase extraction using immobilized-phenylboronic acid (PBA) in sample pr
etreatment, all adenosine derivatives' are retained via their intact c
is-diol group. On-line desorption of the analytes from the PBA support
to the C,, analytical column is performed by injection of a small plu
g of perchloric acid. Fluorescence and UV detection are employed for t
he different adenosine derivatives. The method is applied to the deter
mination of )phenylethylamino]-5'-N-ethylcarboxyamidoadenosine (CGS 21
680, I) in blood using fluorescence detection. The only off-line sampl
e handling step is the extraction of blood with ethyl acetate and subs
equent evaporation of the extraction solvent. The detection limit of t
he method was 0.25 ng (signal-to-noise ratio 3:1) and the determinatio
n limit for I in blood (pretreatment of 100 mu l) was 5 ng/ml. The met
hod was validated and used to study the pharmacokinetics of I in rats.