C. Polanco et Mp. Delavega, THE STRUCTURE OF THE RDNA INTERGENIC SPACER OF AVENA-SATIVA L - A COMPARATIVE-STUDY, Plant molecular biology, 25(4), 1994, pp. 751-756
The sequence of the 18S-25S ribosomal RNA gene intergenic spacer (IGS)
of Avena sativa was determined. DNA was cloned after polymerase chain
reaction amplification of the IGS. The spacer of 3980 bp is composed
of non-repeated sequences and five tandem arrays of repeated sequences
, named A to E. Homology between oats IGS and other grass species was
found. Tandem arrays D and E seem to be originated by duplication from
single-copy sequences in related species. A transcription initiation
site and putative sites of termination, processing and methylation wer
e detected by computer-aided search. These sites resemble motifs conse
rved in the IGS of plant species.