APPLICATION OF MIXED MOBILE PHASES AND A STEP GRADIENT-METHOD IN CAPILLARY ELECTROCHROMATOGRAPHY FOR THE SEPARATION OF ISOMERIC POLYCYCLIC AROMATIC HYDROCARBON-DEOXYRIBONUCLEOSIDE ADDUCT MIXTURES PREPARED IN-VITRO

Capillary electrochromatography (CEC) was used for the analysis of mix tures of neutral isomeric compounds derived from the reaction of carci nogenic hydrocarbon (benzo[g]chrysene and 5,6-dimethylchrysene) dihydr odiol epoxides with calf thymus deoxyribonucleic acid (DNA). The CEC a nalysis demonstrated higher resolution, greater speed and lower analyt e consumption than high-performance liquid chromatography (HPLC) in th e analysis of the same samples using the same type of stationary phase . Proper selection of the mixed mobile phases was critical for the sep aration of these complex mixtures with enhanced speed and selectivity. The use of a step gradient further improved the speed of the CEC anal ysis resulting in electrochromatograms that required only 25-70% of th e corresponding HPLC analysis times. (C) 1997 Elsevier Science B.V.