DNA FRAGMENT ANALYSIS BY CAPILLARY POLYMER SIEVING ELECTROPHORESIS USING POLY(ACRYLOYLAMINOETHOXYETHANOL)-COATED CAPILLARIES

Capillary polymer sieving electrophoresis (CPSE) using dynamic sieving polymer solutions provides a reliable technique for the accurate size identification of doublestranded (ds) DNA fragments. The polymer siev ing systems described here resolve dsDNA fragments in the 100-4000 bas e pair size range. The effect of temperature, capillary length and sie ving polymer concentration were examined. Best performance has been ac hieved with capillaries coated with a new monomer, poly acrylolyaminoe thoxyethanol (AAEE). The poly(AAEE) coating exhibits superior stabilit y at high pH as compared to linear polyacrylamide (LPA). This coating provides the necessary stability to ensure reliable analyses over many runs. (C) 1997 Elsevier Science B.V.