GAS-CHROMATOGRAPHIC SEPARATION OF THE ENANTIOMERS OF VOLATILE FLUOROETHER ANESTHETICS BY DERIVATIZED CYCLODEXTRINS .3. PREPARATIVE-SCALE SEPARATIONS FOR ENFLURANE
Du. Staerk et al., GAS-CHROMATOGRAPHIC SEPARATION OF THE ENANTIOMERS OF VOLATILE FLUOROETHER ANESTHETICS BY DERIVATIZED CYCLODEXTRINS .3. PREPARATIVE-SCALE SEPARATIONS FOR ENFLURANE, Journal of chromatography, 677(1), 1994, pp. 133-140
Preparative-scale gas chromatographic separation of the enantiomers of
enflurane, a volatile anesthetic, has been achieved with a 1 m long,
10 mm I.D. column, packed with 25% (w/w) trifluoroacetyl-gamma-cyclode
xtrin-coated 60/80 mesh Chromosorb A, installed in a custom-designed p
reparative gas chromatograph. An effluent-sampling interface valve, lo
cated between the exit of the preparative column and the fraction coll
ector, takes 10-mu 1 samples of the effluent every 15 s and sends them
onto a short, efficient analytical capillary column providing on-line
enantiomeric analysis of the eluting bands. This permits the precise
calculation of product purity, recovery and production rate for the pr
eparative separation and leads to aggressive, yet safe, fraction pooli
ng schemes.