M. Arellano et al., CAPILLARY ELECTROPHORESIS AND INDIRECT UV DETECTION AS A FAST AND SIMPLE ANALYTICAL TOOL FOR BACTERIAL TAXONOMY, Journal of chromatography, 781(1-2), 1997, pp. 497-501
Citations number
12
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Short-chain methylated fatty acids are difficult to study using conven
tional techniques such as gas chromatography, because of their high vo
latility. Moreover, the treatment of the samples is long and fastidiou
s. Fatty acids are very interesting substances for the characterizatio
n of anaerobic bacteria. In this work we develop a new fast and simple
method using capillary electrophoresis and indirect UV detection for
the analysis of different short-chain fatty acids. We analyzed culture
media of different anaerobic bacteria and found some concentration pa
tterns which are discriminant at the species level. Prior to analysis
the culture media were simply filtrated and diluted. (C) 1997 Elsevier
Science B.V.