BIOCONCENTRATION, ELIMINATION AND METABOLISM OF 2,4-DINITROTOLUENE INCARPS (CYPRINUS-CARPIO L.)

Bioconcentration curves of 2,4-DNT in carps(whole fish, liver, intesti ne) were investigated using semistatic systems. For whole fish, its cu rve could be described as a gentle peak which began with a rise in con centration to steady state, then declined and reached lower level foll owed by another steady state. For liver and intestine, their curves bo th contained two successive peaks. Bioconcentration factors(BCFs) of 2 ,4-DNT in whole fish at the first and second steady state were 9.15 an d 4.15 respectively. By logarithmic plotting, two straight lines with different slopes 3.6 and 0.1 d(-1) were measured for elimination. Two metabolites were isolated by HPLC as 4-amino-2-nitrotoluene and 2,4-di aminotoluene, their retention times were 23.1 and 8.8 min respectively . A one-compartment model was set up to simulate the uptake, in which biotransformation adhered to delayed enzyme-catalytic logarithmic kine tics. Modelling curve was in accord with measured values.