MECHANISM OF VASORELAXATION OF THORACIC AORTA CAUSED BY XANTHONE

The effect of xanthone on smooth muscle was studied in thoracic aorta isolated from rats. Xanthone relaxed the norepinephrine-induced contra ction of rat thoracic aorta. This relaxing effect of xanthone persiste d in endothelium-denuded aorta suggesting that the relaxation induced by xanthone is endothelium-independent. The norepinephrine and high-Ki -induced vasoconstriction was inhibited dose dependently in aorta pret reated with xanthone with IC50 values of 60.26 +/- 8.43 and 82.9 +/- 1 3.21 mu M, respectively. The inositol 1,4,5-trisphosphate formation in duced by norepinephrine (3 mu M) in rat aorta was not affected by xant hone (10-100 mu M), suggesting that the vasorelaxant effect of xanthon e was not exerted on the receptor. Xanthone concentration dependently inhibited the Ca-45(2+) influx induced by either norepinephrine or hig h-K+ suggesting that xanthone might act as a blocker of both receptor- operated and voltage-dependent Ca2+ channels. Furthermore, xanthone ca used an increase in the level of intracellular cyclic adenosine 3',5'- monophosphate (cAMP), but not cyclic guanosine 3',5'-monophosphate (cG MP) content. These data suggested that the mechanism of xanthone-induc ed vasorelaxation might involve the increase of intracellular cyclic a denosine 3',5' monophosphate (CAMP) content and block of Ca2+ channels . (C) 1997 Elsevier Science B.V.