EFFECT OF HUMAN HYDROSALPINX FLUID ON MURINE EMBRYO DEVELOPMENT AND IMPLANTATION

Objective: To determine the effect of hydrosalpinx fluid-containing me dium on murine embryo development and implantation. Design: The develo pment of one-, two-, and four-cell mouse embryos in medium containing 5%, 10%, and 20% of human hydrosalpinx fluid was observed. Implantatio n rates of mouse embryos transferred into the uterine horn with hydros alpinx fluid-containing media were determined. Setting: Private hospit al-based fertility center and IVF program. Main Outcome Measure(s): Pe rcentage of embryos continuing cell division and implantation rates af ter ET. Result(s): Hydrosalpinx fluid in culture medium affected embry o development in a dose-dependent fashion. The injection of hydrosalpi nx fluid-containing medium into the uterine horn did not affect embryo implantation. Conclusion(s): Hydrosalpinx fluid negatively affects mu rine embryo development, but its presence in the uterine horn at ET di d not affect implantation. (C) 1997 by American Society for Reproducti ve Medicine.