CATALYTIC MECHANISM OF KDO8P SYNTHASE - PRE-STEADY-STATE KINETIC-ANALYSIS USING RAPID CHEMICAL QUENCH FLOW METHODS

Authors
LIANG PH KOHEN A BAASOV T ANDERSON KS
Citation
Ph. Liang et al., CATALYTIC MECHANISM OF KDO8P SYNTHASE - PRE-STEADY-STATE KINETIC-ANALYSIS USING RAPID CHEMICAL QUENCH FLOW METHODS, Bioorganic & medicinal chemistry letters, 7(19), 1997, pp. 2463-2468
Citations number
26
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
7
Issue
19
Year of publication
1997
Pages
2463 - 2468
Database
ISI
SICI code
0960-894X(1997)7:19<2463:CMOKS->2.0.ZU;2-8
Abstract
The catalytic mechanism of 3-deoxy-D-manno-2-octulosonate-8-phosphate (Kdo8P) synthase from Escherichia coli was investigated under pre-stea dy-state conditions using rapid chemical quench flow methods. The resu lts suggest the formation of acyclic bisphosphate 1 as a reaction inte rmediate. (C) 1997 Elsevier Science Ltd.