DETERMINATION OF PROGESTERONE IN NANOCAPSULES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

In order to determine progesterone concentration in nanocapsules consi sting of benzyl benzoate in a poly-epsilon-caprolactone shell, various methods were tested for preparation of samples. The opening of nanoca psules by a dissolution-dilution in acetonitrile was chosen. The metho d was validated. A reversed phase HPLC method using an acetonitrile-wa ter mixture (75:25) as mobile phase, demonstrated that the entrapping of progesterone was almost total in the nanocapsules, and that, only t races of progesterone were present in the aqueous phase of the suspens ion. The lack of adsorption on the polymeric wall was also demonstrate d.