C. Bouniolbaly et al., DYNAMIC ORGANIZATION OF DNA-REPLICATION IN ONE-CELL MOUSE EMBRYOS - RELATIONSHIP TO TRANSCRIPTIONAL ACTIVATION, Experimental cell research, 236(1), 1997, pp. 201-211
We have analyzed the spatial and temporal relationship between transcr
iption and replication sites during the first cell cycle in mouse embr
yos. Embryos were microinjected with both 5-bromouridine-5'-triphospha
te and digoxygenin-11-deoxyuridine-5'-triphosphate to visualize transc
ription and replication sites respectively. We detected six different
phases of replication during S phase and dated the onset of zygotic tr
anscription at the end of the S phase. Using confocal microscopy, we s
howed that there is essentially no colocalization of replication and t
ranscription sites at this stage of development. Moreover, studies on
aphidicolin-treated embryos demonstrated that inhibition of DNA replic
ation does not hinder transcriptional activation at the 1-cell stage.
(C) 1997 Academic Press.