MOLECULAR-CLONING OF RAT G-PROTEIN-COUPLED-RECEPTOR-KINASE-6 (GRK6) FROM BRAIN-TISSUE, AND ITS MESSENGER-RNA EXPRESSION IN DIFFERENT BRAIN-REGIONS AND PERIPHERAL-TISSUES

Authors
FEHR C FICKOVA M HIEMKE C REUSS S DAHMEN N
Citation
C. Fehr et al., MOLECULAR-CLONING OF RAT G-PROTEIN-COUPLED-RECEPTOR-KINASE-6 (GRK6) FROM BRAIN-TISSUE, AND ITS MESSENGER-RNA EXPRESSION IN DIFFERENT BRAIN-REGIONS AND PERIPHERAL-TISSUES, Molecular brain research, 49(1-2), 1997, pp. 278-282
Citations number
25
Categorie Soggetti
Neurosciences
Journal title
Molecular brain research → ACNP
ISSN journal
0169328X
Volume
49
Issue
1-2
Year of publication
1997
Pages
278 - 282
Database
ISI
SICI code
0169-328X(1997)49:1-2<278:MORG(F>2.0.ZU;2-8
Abstract
The rat G-protein-coupled receptor kinase 6 (GRK6) cDNA was cloned fro m rat brain tissue by a combination of reverse-transcription polymeras e chain reactions (RT-PCR), based on homology to the cloned human GRK6 , and rapid amplification of cDNA ends (RACE-PCR). We obtained a clone of 2817 bp with an open reading frame of 1731 bp encoding for a prote in of 576 amino acids that is 96.7% identical and 97.9% similar to its human counterpart. mRNA was detectable in all brain areas examined. I n addition, GRK6 was expressed in skeletal muscle, small intestine, ao rta, liver, heart, lung, thymus, stomach, uterus and kidney. (C) 1997 Elsevier Science B.V.