MOLECULAR-CLONING AND CHROMOSOMAL LOCALIZATION OF PD-I-BETA (PDNP3), A NEW MEMBER OF THE HUMAN PHOSPHODIESTERASE-I GENES

Phosphodiesterase I (EC 3.1.4.1)/nucleotide pyrophosphatase (EC 3.6.1. 9) enzymes are a family of type II transmembrane proteins that catalyz e the cleavage of phosphodiester and phosphosulfate bonds of a variety of molecules, including deoxynucleotides, NAD, and nucleotide sugars. The human genes for two members of this family have been cloned and d esignated PC-1 (PDNP1) and PD-I alpha/autotaxin (PDNP2). We have now c loned the third member of this family from a human prostate cDNA libra ry and designated it human phosphodiesterase-I beta (PD-I beta). The P D-I beta cDNA contains a 2625-bp-long open reading frame which encodes an 875-amino-acid protein. COS-7 cells transfected with an expression vector, pBK-CMV, containing PD-I beta cDNA had high phosphodiesterase I activity compared to the mock-transfected cells. By using in situ h ybridization to human metaphase chromosomes, we have assigned the locu s for the PD-I beta (PDNP3) gene to the q22 region of human chromosome 6. (C) 1997 Academic Press.