CHARACTERIZATION OF A KRAB FAMILY ZINC-FINGER GENE, ZNF195, MAPPING TO CHROMOSOME BAND 11P15.5

We report the cDNA sequence of the zinc finger gene, ZNF195, which map s to chromosome 11p15.5. ZNF195 contains an N-terminal KRAB domain and 14 tandemly repeated Kruppel type zinc finger motifs at its C-terminu s. Northern analysis shows expression of ZNF195 in adult heart, brain, placenta, skeletal muscle, and pancreas with a predominant transcript size of 4.3 kb. There is little expression in adult lung, liver, and kidney. In fetal lung, liver, kidney, and brain, the predominant trans cript is 3.5 kb. Fetal brain also expresses a 4.3-kb transcript. RT-PC R analysis shows that two exons, 4a, which contains an inverted Alu se quence, and 4b, are differentially spliced and absent from the major t ranscript. (C) 1997 Academic Press.