CLONING AND EXPRESSION OF A CDNA-ENCODING BOVINE MUSCARINIC ACETYLCHOLINE M3 RECEPTOR

Authors
LEE PHK HODGES PK GLICKMAN F CHANG KJ
Citation
Phk. Lee et al., CLONING AND EXPRESSION OF A CDNA-ENCODING BOVINE MUSCARINIC ACETYLCHOLINE M3 RECEPTOR, Biochimica et biophysica acta. Molecular cell research, 1223(1), 1994, pp. 151-154
Citations number
11
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta. Molecular cell research → ACNP
ISSN journal
01674889
Volume
1223
Issue
1
Year of publication
1994
Pages
151 - 154
Database
ISI
SICI code
0167-4889(1994)1223:1<151:CAEOAC>2.0.ZU;2-N
Abstract
A cDNA clone encoding bovine muscarinic acetylcholine m3 receptor has been isolated from a bovine brain library. Sequencing of this clone ha s revealed a single open reading frame encoding a protein of 590 amino acids. Comparison with known muscarinic m3 receptor sequences from ot her species shows a high degree of conservation (92-98% homology) in t he protein sequence. COS cells transfected with this cDNA expressed a single high-affinity (K-d = 43.9 pM) binding site for muscarinic recep tor ligand N-[H-3]methylscopolamine.