THE LONG-CHAIN FATTY-ACIDS OF PASTEURELLA -MULTOCIDA AND PASTEURELLA-HAEMOLYTICA

The relative contents of long-chain fatty acids in P. multocida and P. haemolytica were investigated. A dependence on the composition of the broth was established. Accordingly, comparative quantitative studies on fatty acid contents have to be conducted using bacteria grown with the same lot of broth medium. As for P. multocida, there were signific ant differences between the serovars (C14 in TDHM and C16, Delta(2)C18 in BPL). These differences are, however, not significant to replace s erotyping. Highly significant differences were also detected between e . multocida isolates from nasal swabs and pneumonic lungs (interims of C14, Delta C16 on BPL and BRU). The largest differences were measured for strains grown on BRU, which is interpreted as an expression of vi rulence. Significant differences were found between biotypes A and T o f P. haemolytica,, namely for C14, C16 in TDHM, and C14, Delta C16, C1 6, C18 in BPL medium.