IDENTIFICATION AND CHARACTERIZATION OF A PUTATIVE SERINE-PROTEASE EXPRESSED IN-VIVO BY MYCOBACTERIUM-AVIUM SUBSP PARATUBERCULOSIS

Authors
CAMERON RM STEVENSON K INGLIS NF KLAUSEN J SHARP JM
Citation
Rm. Cameron et al., IDENTIFICATION AND CHARACTERIZATION OF A PUTATIVE SERINE-PROTEASE EXPRESSED IN-VIVO BY MYCOBACTERIUM-AVIUM SUBSP PARATUBERCULOSIS, Microbiology, 140, 1994, pp. 1977-1982
Citations number
40
Categorie Soggetti
Microbiology
Journal title
Microbiology → ACNP
ISSN journal
13500872
Volume
140
Year of publication
1994
Part
8
Pages
1977 - 1982
Database
ISI
SICI code
1350-0872(1994)140:<1977:IACOAP>2.0.ZU;2-R
Abstract
A putative serine protease expressed in vivo by Mycobacterium avium su bsp. paratuberculosis was isolated from a lambda gt11 genomic expressi on library by screening with serum from a naturally infected sheep. Th e gene was contained in two overlapping clones. which were shown by an tibody elution to encode a protein of 34 kDa in M. a. paratuberculosis . The clones were sequenced and database searches detected a motif ide ntical to the active serine site in trypsin, and 30% homology to the p utative serine proteases (HtrA proteins) of Escherichia coil, Salmonel la typhimurium, Brucella abortus and Rochalimaea henselae.