IMMUNOLOGICAL DETECTION OF SHEEP EXPERIMENTALLY INFECTED WITH STRAINSOF MYCOBACTERIUM-AVIUM SUBSPECIES CONTAINING INSERTION-SEQUENCE IS901IS902 AND A 40 KDA PROTEIN/

Authors
KLAUSEN J PEREZ V GIESE SB MARIN JFG AHRENS P
Citation
J. Klausen et al., IMMUNOLOGICAL DETECTION OF SHEEP EXPERIMENTALLY INFECTED WITH STRAINSOF MYCOBACTERIUM-AVIUM SUBSPECIES CONTAINING INSERTION-SEQUENCE IS901IS902 AND A 40 KDA PROTEIN/, Veterinary microbiology, 57(2-3), 1997, pp. 181-187
Citations number
19
Categorie Soggetti
Microbiology,"Veterinary Sciences
Journal title
Veterinary microbiology → ACNP
ISSN journal
03781135
Volume
57
Issue
2-3
Year of publication
1997
Pages
181 - 187
Database
ISI
SICI code
0378-1135(1997)57:2-3<181:IDOSEI>2.0.ZU;2-#
Abstract
A monoclonal antibody raised against a 40 kDa protein present in certa in M. avium strains (IS901/IS902 positive) was used for developing a b locking ELISA. Sera from experimentally infected sheep were evaluated by indirect ELISA, AGID and blocking ELISA. The blocking assay proved to be highly specific for differentiation of sheep infected with diffe rent subspecies of M. avium. (C) 1997 Elsevier Science B.V.